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Image Search Results
Journal: bioRxiv
Article Title: The Aedes aegypti peritrophic matrix controls arbovirus vector competence through HPx1, a heme–induced peroxidase
doi: 10.1101/2022.06.02.494599
Figure Lengend Snippet: The role of HPx1 in PM assembly. A) Midgut transverse slices at 18 h ABM supplemented with dextran. Green: dextran – FITC. Blue: DAPI nuclear staining. Insets highlight dextran localization. B) Representative images of ROS levels measured by DHE oxidation in individual midguts at 18 h ABM. B’) Quantitative analysis of the fluorescence intensity of oxidized DHE (dsLacZ, n=35; dsHPx1, n=39). C) Quantitative analysis of the fluorescence intensity of oxidized DHE from individual midguts at 18 h ABM (dsLacZ, n=32; dsHPx1 - Ab, n=34; dsHPx1 + Ab, n=27). D) Mitosis index in the mosquito midgut at 18 h ABM (phospho-histone H3) (dsLacZ n=33; dsHPx1 n=38). E) The intestinal microbiota load analyzed through eubacterial ribosomal 16S gene expression by qPCR at 24 h ABM (dsLacZ n=6; dsHPx1 n=7). F) Immune-related gene expression upon HPx1 silencing at 24 h ABM by qPCR (dsLacZ n=8; dsHPx1 n=7). *p<0.05, ****p<0.0001, ns = not significant. Data are the mean +/-SEM. The T test for B’, D, E and F, and one-way ANOVA with Tukey’s posttest for C.
Article Snippet: All samples were incubated overnight with a
Techniques: Staining, Fluorescence, Gene Expression